Hotspot

psbM-trnD-GUC

LSC · 31131-32162 · 1032 bp

Back to Sternbergia

Pi 0.0111

MarkerSeek score 62.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1032
variable_sites: 11
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	CCTCGCTGGGATTGTAGTTC	45.0	55.0	55.8	58.1	1128-1160 bp	1.0	65.1
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	AGTTCAATCGGTCAGAGCAC	45.0	50.0	55.8	57.9	1113-1145 bp	1.0	64.9
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	TACACCTCGCTGGGATTGTA	45.0	50.0	55.8	58.1	1132-1164 bp	1.0	64.4
psbM-trnD-GUC_p4	AGGAACTAGAATGAACAGTGCA	CCTCGCTGGGATTGTAGTTC	40.9	55.0	57.4	58.1	1135-1167 bp	1.0	61.9
psbM-trnD-GUC_p5	AGGAACTAGAATGAACAGTGCA	AGTTCAATCGGTCAGAGCAC	40.9	50.0	57.4	57.9	1120-1152 bp	1.0	61.7