Hotspot

petN-psbM

LSC · 30051-31025 · 975 bp

Back to Sternbergia

Pi 0.0114
MarkerSeek score 63.10
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
975
variable_sites
11
pi
0.0114
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCTTGCTTGGGCTGCTTTAA TGCTACTGCACTGTTCATTCT 45.0 42.9 58.0 57.6 1127-1130 bp 1.0 70.0
petN-psbM_p2 AGTATGGGGAAGGAGTGGAC TGCTACTGCACTGTTCATTCT 55.0 42.9 58.1 57.6 1076-1079 bp 1.0 69.2
petN-psbM_p3 GTATGGGGAAGGAGTGGACT TGCTACTGCACTGTTCATTCT 55.0 42.9 58.1 57.6 1075-1078 bp 1.0 69.2
petN-psbM_p4 ATGGTAAGTCTTGCTTGGGC TGCTACTGCACTGTTCATTCT 50.0 42.9 58.2 57.6 1135-1138 bp 1.0 68.8
petN-psbM_p5 TATGGGGAAGGAGTGGACTC TGCTACTGCACTGTTCATTCT 55.0 42.9 57.8 57.6 1074-1077 bp 1.0 68.7