Hotspot

trnS-GCU-trnG-GCC

LSC · 8598-9855 · 1258 bp

Back to Sternbergia

Pi 0.0209

MarkerSeek score 65.70

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1258
variable_sites: 25
pi: 0.0209
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ATTAGCAATCCGCCGCTTTA	CGAACGAATCACACTTTTACCA	45.0	40.9	58.0	57.3	1335-1345 bp	1.0	63.2
trnS-GCU-trnG-GCC_p2	ATTAGCAATCCGCCGCTTTA	TCGAACGAATCACACTTTTACC	45.0	40.9	58.0	57.0	1336-1346 bp	1.0	61.1
trnS-GCU-trnG-GCC_p3	CGGAGAGAGAGGGATTCGAA	CGAACGAATCACACTTTTACCA	55.0	40.9	58.3	57.3	1383-1393 bp	1.0	60.8
trnS-GCU-trnG-GCC_p4	GCTTTAGTCCACTCAGCCAT	CGAACGAATCACACTTTTACCA	50.0	40.9	57.6	57.3	1321-1331 bp	1.0	60.3
trnS-GCU-trnG-GCC_p5	AGTCCACTCAGCCATCTCTC	CGAACGAATCACACTTTTACCA	55.0	40.9	58.5	57.3	1316-1326 bp	1.0	59.5