Hotspot

psbA-trnK-UUU

LSC · 1154-1390 · 237 bp

Back to Sternbergia

Pi 0.0295

MarkerSeek score 63.70

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 237
variable_sites: 7
pi: 0.0295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	AAAACACCGAACCATCCGAT	AAAATGCAAGCACGGTTTGG	45.0	45.0	57.8	58.4	448-449 bp	1.0	76.3
psbA-trnK-UUU_p2	TAAAGACGGTTTTCGGTGCT	AAAATGCAAGCACGGTTTGG	45.0	45.0	57.8	58.4	427-428 bp	1.0	76.0
psbA-trnK-UUU_p3	AAAACACCGAACCATCCGAT	CTTCATCCGACTAGTTCCGG	45.0	55.0	57.8	57.5	385-386 bp	1.0	75.9
psbA-trnK-UUU_p4	GCTTGTACTTTCGCGTCTCT	AAAATGCAAGCACGGTTTGG	50.0	45.0	58.3	58.4	377-378 bp	1.0	75.7
psbA-trnK-UUU_p5	CACCGAACCATCCGATGTAA	AAAATGCAAGCACGGTTTGG	50.0	45.0	57.7	58.4	444-445 bp	1.0	75.5