Hotspot

rps3

LSC · 82371-83024 · 654 bp

Back to Sterigmostemum

Pi 0.00153

MarkerSeek score 59.60

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 654
variable_sites: 1
pi: 0.00153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	AAAAAGAAGGCTGCATTTTT	AAACCTGGATGCAGCAATGA	30.0	45.0	52.6	58.1	809 bp	1.0	40.1
rps3_p2	AAAAAGAAGGCTGCATTTTT	AACCTGGATGCAGCAATGAA	30.0	45.0	52.6	58.1	808 bp	1.0	40.1
rps3_p3	AAAAAGAAGGCTGCATTTTT	ACCTGGATGCAGCAATGAAA	30.0	45.0	52.6	58.1	807 bp	1.0	40.1
rps3_p4	AAAAAGAAGGCTGCATTTTT	AAAACCTGGATGCAGCAATG	30.0	45.0	52.6	57.2	810 bp	1.0	40.1
rps3_p5	AAAAAGAAGGCTGCATTTTT	CCTGGATGCAGCAATGAAAA	30.0	45.0	52.6	57.0	806 bp	1.0	40.1