Hotspot

rpl16

LSC · 80808-82203 · 1396 bp

Back to Sterigmostemum

Pi 0.00144

MarkerSeek score 60.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1396
variable_sites: 2
pi: 0.00144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCTTTTAATGCAAAGGACGAAGT	TTTCCATCAAAACCCCTTTG	34.8	40.0	57.0	54.2	1526-1531 bp	1.0	40.4
rpl16_p2	CTCTTCTTTTAATGCAAAGGACGA	TTTCCATCAAAACCCCTTTG	37.5	40.0	57.8	54.2	1530-1535 bp	1.0	40.4
rpl16_p3	TCTCTTCTTTTAATGCAAAGGACG	TTTCCATCAAAACCCCTTTG	37.5	40.0	57.8	54.2	1531-1536 bp	1.0	40.4
rpl16_p4	TCTTCTTTTAATGCAAAGGACGA	TTTCCATCAAAACCCCTTTG	34.8	40.0	56.7	54.2	1529-1534 bp	1.0	40.4
rpl16_p5	TTCTTTTAATGCAAAGGACGAAGT	TTTCCATCAAAACCCCTTTG	33.3	40.0	57.6	54.2	1527-1532 bp	1.0	40.4