Hotspot

atpI

LSC · 13555-14304 · 750 bp

Back to Sterigmostemum

Pi 0

MarkerSeek score 29.20

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: LSC
length_bp: 750
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	TGGGCTAACCTAAAAACGAA	AGTTCTTATTTATGTCAGAGGGCA	40.0	37.5	54.9	57.7	809 bp	1.0	40.0
atpI_p2	TTGGGCTAACCTAAAAACGA	AGTTCTTATTTATGTCAGAGGGCA	40.0	37.5	54.9	57.7	810 bp	1.0	40.0
atpI_p3	TTGGGCTAACCTAAAAACGAA	AGTTCTTATTTATGTCAGAGGGCA	38.1	37.5	55.7	57.7	810 bp	1.0	40.0
atpI_p4	TGGGCTAACCTAAAAACGAA	GTTCTTATTTATGTCAGAGGGCA	40.0	39.1	54.9	56.3	808 bp	1.0	40.0
atpI_p5	TTGGGCTAACCTAAAAACGA	GTTCTTATTTATGTCAGAGGGCA	40.0	39.1	54.9	56.3	809 bp	1.0	40.0