Hotspot

atpA

LSC · 9496-11019 · 1524 bp

Back to Sterigmostemum

Pi 0.00197

MarkerSeek score 59.80

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 3
pi: 0.00197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	AGCCTTCCAAGCTAACGATG	AATCGGGAGAGGATAGGCTC	50.0	55.0	58.0	58.1	2729 bp	1.0	79.3
atpA_p2	AGCCTTCCAAGCTAACGATG	ATCAGTTTCGCGTGAATGGA	50.0	45.0	58.0	57.9	2422 bp	1.0	79.1
atpA_p3	AGCCTTCCAAGCTAACGATG	TCAGTTTCGCGTGAATGGAT	50.0	45.0	58.0	57.9	2421 bp	1.0	79.1
atpA_p4	AGCCTTCCAAGCTAACGATG	TCATGTTTGGTTCGGGAAGG	50.0	50.0	58.0	58.1	2619 bp	1.0	79.1
atpA_p5	CCTAGCCTTCCAAGCTAACG	AATCGGGAGAGGATAGGCTC	55.0	55.0	58.1	58.1	2732 bp	1.0	79.1