Hotspot

rpl32-trnL-CUA

SSC · 113202-113670 · 469 bp

Back to Sterigmostemum

Pi 0.0171
MarkerSeek score 65.80
Primer available yes
Label rpl32-trnL-CUA

Feature Metadata

label_name
rpl32-trnL-CUA
feature_type
igs
region
SSC
length_bp
469
variable_sites
8
pi
0.0171
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-CUA_p1 GGAAAAGGAAGGGATATTGGACA TCCTAAGAGCAGCGTGTCTA 43.5 50.0 58.1 57.9 609 bp 1.0 55.0
rpl32-trnL-CUA_p2 TGGAAAAGGAAGGGATATTGGAC TCCTAAGAGCAGCGTGTCTA 43.5 50.0 58.1 57.9 610 bp 1.0 55.0
rpl32-trnL-CUA_p3 GGAAAAGGAAGGGATATTGGACA AGCAGCGTGTCTACCAATTT 43.5 45.0 58.1 57.8 602 bp 1.0 54.5
rpl32-trnL-CUA_p4 TGGAAAAGGAAGGGATATTGGAC AGCAGCGTGTCTACCAATTT 43.5 45.0 58.1 57.8 603 bp 1.0 54.5
rpl32-trnL-CUA_p5 AGGAAGGGATATTGGACATCA TCCTAAGAGCAGCGTGTCTA 42.9 50.0 55.8 57.9 604 bp 1.0 54.4