Hotspot

ndhF-rpl32

SSC · 112161-113042 · 882 bp

Back to Sterigmostemum

Pi 0
MarkerSeek score 50.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
882
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.41
markerseek_score
50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAATGAAAGGGA ACGAATACGCTTTTTCGAGA 47.6 40.0 57.6 55.5 459-982 bp 1.0 52.9
ndhF-rpl32_p2 CCACATACGCCGAAGATTTT ACGAATACGCTTTTTCGAGA 45.0 40.0 56.5 55.5 527-1050 bp 1.0 52.5
ndhF-rpl32_p3 TTGTTGCTGTCGGAAAAAGT ACGAATACGCTTTTTCGAGA 40.0 40.0 56.4 55.5 507-1030 bp 1.0 51.5
ndhF-rpl32_p4 TGGAAGTGGAATGAAAGGGA ACGAATACGCTTTTTCGAGA 45.0 40.0 56.3 55.5 458-981 bp 1.0 51.0
ndhF-rpl32_p5 CCACATACGCCGAAGATTTTT ACGAATACGCTTTTTCGAGA 42.9 40.0 57.2 55.5 527-1050 bp 1.0 50.3