Hotspot

psaJ-rpl33

LSC · 66612-67021 · 410 bp

Back to Sterigmostemum

Pi 0.00978
MarkerSeek score 50.10
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
410
variable_sites
4
pi
0.00978
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TCCAGATGCATTAACATTTCCCT ATATCTGGAAATTCCCGCCG 39.1 50.0 58.1 57.8 539-540 bp 1.0 54.2
psaJ-rpl33_p2 TCCAGATGCATTAACATTTCCC ATATCTGGAAATTCCCGCCG 40.9 50.0 56.7 57.8 539-540 bp 1.0 53.1
psaJ-rpl33_p3 CCAGATGCATTAACATTTCCCT ATATCTGGAAATTCCCGCCG 40.9 50.0 56.7 57.8 538-539 bp 1.0 53.0
psaJ-rpl33_p4 TCCAGATGCATTAACATTTCCCT AATTCCCGCCGATTCTTTCT 39.1 45.0 58.1 57.6 530-531 bp 1.0 52.4
psaJ-rpl33_p5 TCCAGATGCATTAACATTTCCCT ATTCCCGCCGATTCTTTCTT 39.1 45.0 58.1 57.6 529-530 bp 1.0 52.4