Hotspot

psbE-petL

LSC · 64208-65289 · 1082 bp

Back to Sterigmostemum

Pi 0.00185

MarkerSeek score 60.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1082
variable_sites: 2
pi: 0.00185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGA	45.0	45.0	57.9	56.3	1233-1243 bp	1.0	66.0
psbE-petL_p2	AACCAGCCCGCAATGAATAA	AGCTGCTAGTAGAAAACCGA	45.0	45.0	58.2	56.3	1231-1241 bp	1.0	65.3
psbE-petL_p3	AATAACCAGCCCGCAATGAA	AGCTGCTAGTAGAAAACCGA	45.0	45.0	58.2	56.3	1234-1244 bp	1.0	65.3
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1151-1161 bp	1.0	65.3
psbE-petL_p5	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGA	55.0	45.0	58.2	56.3	1146-1156 bp	1.0	65.0