Hotspot

psbK-psbI

LSC · 7044-7301 · 258 bp

Back to Sterigmostemum

Pi 0.00389

MarkerSeek score 55.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 258
variable_sites: 1
pi: 0.00389
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCGGCTGTAAGTTTTCG	TTCTTCACGTCCCGGATTAC	50.0	50.0	58.0	57.3	389-390 bp	1.0	74.7
psbK-psbI_p2	AGCGGCTGTAAGTTTTCGAT	TTCTTCACGTCCCGGATTAC	45.0	50.0	57.9	57.3	387-388 bp	1.0	74.4
psbK-psbI_p3	AAGCGGCTGTAAGTTTTCGA	TTCTTCACGTCCCGGATTAC	45.0	50.0	58.1	57.3	388-389 bp	1.0	74.1
psbK-psbI_p4	GCGGCTGTAAGTTTTCGATG	TTCTTCACGTCCCGGATTAC	50.0	50.0	57.8	57.3	386-387 bp	1.0	73.9
psbK-psbI_p5	GCAAGCGGCTGTAAGTTTTC	TTCTTCACGTCCCGGATTAC	50.0	50.0	58.3	57.3	390-391 bp	1.0	72.6