Hotspot

trnK-AAA-rps16

LSC · 4286-4884 · 599 bp

Back to Sterigmostemum

Pi 0.00167

MarkerSeek score 59.70

Primer available yes

Label trnK-AAA-rps16

Feature Metadata

label_name: trnK-AAA-rps16
feature_type: igs
region: LSC
length_bp: 599
variable_sites: 1
pi: 0.00167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-AAA-rps16_p1	AAAGCCGAGTACTCTACCGT	CGCTCAACCAACAAGAACAG	50.0	50.0	58.2	57.6	703 bp	1.0	75.6
trnK-AAA-rps16_p2	AAGCCGAGTACTCTACCGTT	CGCTCAACCAACAAGAACAG	50.0	50.0	58.2	57.6	702 bp	1.0	75.6
trnK-AAA-rps16_p3	TACCGTTGAGTTAGCAACCC	CGCTCAACCAACAAGAACAG	50.0	50.0	57.5	57.6	689 bp	1.0	73.3
trnK-AAA-rps16_p4	AGTCGCACTTAAAAGCCGAG	CGCTCAACCAACAAGAACAG	50.0	50.0	58.6	57.6	714 bp	1.0	72.4
trnK-AAA-rps16_p5	AAGCCGAGTACTCTACCGTT	AGGCAGGGATTTTTACGGAA	50.0	45.0	58.2	57.1	664 bp	1.0	71.6