Hotspot

rpl32-trnL-UAG

SSC · 120661-121752 · 1092 bp

Back to Sterculia

Pi 0.0247
MarkerSeek score 55.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1092
variable_sites
40
pi
0.0247
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGGCGTTGAAAGCTTTTTCA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 1207-1232 bp 1.0 82.1
rpl32-trnL-UAG_p2 CGGCGTTGAAAGCTTTTTCA CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 1208-1233 bp 1.0 82.1
rpl32-trnL-UAG_p3 CGGCGTTGAAAGCTTTTTCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.2 57.9 1217-1242 bp 1.0 81.2
rpl32-trnL-UAG_p4 CGGCGTTGAAAGCTTTTTCA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 58.2 57.8 1216-1241 bp 1.0 80.6
rpl32-trnL-UAG_p5 CGGCGTTGAAAGCTTTTTCA CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.2 57.8 1215-1240 bp 1.0 80.6