Hotspot

rps19-rpl2

IRb · 91331-91382 · 52 bp

Back to Sterculia

Pi 0.0897

MarkerSeek score 61.30

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 52
variable_sites: 7
pi: 0.0897
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGGTAGATGCTCTGGACCAA	AGCAAAGTTTGGGTAGAGCC	50.0	50.0	58.0	58.1	397-447 bp	1.0	80.6
rps19-rpl2_p2	CTGCGGCTTGACTAATGACT	AGCAAAGTTTGGGTAGAGCC	50.0	50.0	58.0	58.1	897-947 bp	1.0	80.5
rps19-rpl2_p3	CTGCGGCTTGACTAATGACT	ATTACCTTCTGGGGAGGTCC	50.0	55.0	58.0	58.1	977-1027 bp	1.0	80.2
rps19-rpl2_p4	CTGCGGCTTGACTAATGACT	TGGGGAGGTCCGTTTGATAT	50.0	50.0	58.0	58.1	968-1018 bp	1.0	80.2
rps19-rpl2_p5	CTGCGGCTTGACTAATGACT	TAACATGAGTGGTTCGGCAG	50.0	50.0	58.0	57.9	1323-1373 bp	1.0	80.1