Hotspot

rpl14-rpl16

LSC · 87954-88072 · 119 bp

Back to Sterculia

Pi 0.0226

MarkerSeek score 55.10

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 119
variable_sites: 4
pi: 0.0226
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ATCGGCGATTACTAGCTCCT	AATAGCGGCGTCAAAAATGC	50.0	45.0	58.1	58.1	265-270 bp	1.0	80.1
rpl14-rpl16_p2	ATCGGCGATTACTAGCTCCT	ATGAAATGAGCGGAGTAGCC	50.0	50.0	58.1	57.8	311-316 bp	1.0	78.6
rpl14-rpl16_p3	TACACATCAATTCTCGGGCC	AATAGCGGCGTCAAAAATGC	50.0	45.0	57.7	58.1	235-240 bp	1.0	78.3
rpl14-rpl16_p4	TACACATCAATTCTCGGGCC	ATGAAATGAGCGGAGTAGCC	50.0	50.0	57.7	57.8	281-286 bp	1.0	76.8
rpl14-rpl16_p5	CCCGCTGTTATCTGCTACAT	AATAGCGGCGTCAAAAATGC	50.0	45.0	57.5	58.1	216-221 bp	1.0	76.8