Hotspot

atpB-rbcL

LSC · 60079-60999 · 921 bp

Back to Sterculia

Pi 0.0249
MarkerSeek score 48.80
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
921
variable_sites
34
pi
0.0249
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 CGGTTCATTCATCTGACCGT TTCGCAGATCCTCTAGACGT 50.0 50.0 58.0 58.0 2065-2142 bp 1.0 81.9
atpB-rbcL_p2 CGGTTCATTCATCTGACCGT GTACCCGCAGTAGCATTCAA 50.0 50.0 58.0 58.0 2384-2461 bp 1.0 81.8
atpB-rbcL_p3 TGAATAGGGGATGTTGTGCG GTACCCGCAGTAGCATTCAA 50.0 50.0 58.0 58.0 2056-2133 bp 1.0 81.8
atpB-rbcL_p4 CTGGCGGTTCATTCATCTGA GTACCCGCAGTAGCATTCAA 50.0 50.0 58.0 58.0 2388-2465 bp 1.0 81.7
atpB-rbcL_p5 CGGTTCATTCATCTGACCGT CTCTCCAGCGCATAAATGGT 50.0 50.0 58.0 58.0 2293-2370 bp 1.0 81.7