Hotspot

trnT-UGU-trnL-UAA

LSC · 50029-51997 · 1969 bp

Back to Sterculia

Pi 0.0382

MarkerSeek score 49.30

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1969
variable_sites: 57
pi: 0.0382
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.50
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	55.0	50.0	57.9	58.0	1080-2019 bp	1.0	86.2
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	1088-2027 bp	1.0	85.6
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CCAAGGCTCAATCCAATCCA	55.0	50.0	57.9	57.9	1118-2057 bp	1.0	84.9
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	ACCAAGGCTCAATCCAATCC	55.0	50.0	57.9	57.9	1119-2058 bp	1.0	84.9
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	1094-2033 bp	1.0	84.3