Hotspot

pafI-trnS-GGA

LSC · 47300-48305 · 1006 bp

Back to Sterculia

Pi 0.0227

MarkerSeek score 61.60

Primer available yes

Label pafI-trnS-GGA

Feature Metadata

label_name: pafI-trnS-GGA
feature_type: igs
region: LSC
length_bp: 1006
variable_sites: 33
pi: 0.0227
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafI-trnS-GGA_p1	ACTTCACCTTCAACAAGCGT	GCGAGATTTTCATCCCCTGT	45.0	50.0	58.0	58.0	1495-1524 bp	1.0	84.0
pafI-trnS-GGA_p2	TGAGGTTGCATTAATCGGGG	GCGAGATTTTCATCCCCTGT	50.0	50.0	57.9	58.0	1547-1576 bp	1.0	83.8
pafI-trnS-GGA_p3	ACTTCACCTTCAACAAGCGT	TTCGATTGGGTATGGCTTCG	45.0	50.0	58.0	58.1	1942-1977 bp	1.0	83.3
pafI-trnS-GGA_p4	TGAGGTTGCATTAATCGGGG	TTCGATTGGGTATGGCTTCG	50.0	50.0	57.9	58.1	1994-2029 bp	1.0	83.2
pafI-trnS-GGA_p5	TGAGCGGGGCTAATGTTTTT	GCGAGATTTTCATCCCCTGT	45.0	50.0	58.1	58.0	1955-1983 bp	1.0	82.7