Hotspot

trnD-GUC-trnY-GUA

LSC · 31562-32070 · 509 bp

Back to Sterculia

Pi 0.028

MarkerSeek score 55.70

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 509
variable_sites: 21
pi: 0.028
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	573-579 bp	1.0	78.4
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	572-578 bp	1.0	78.4
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	594-600 bp	1.0	77.4
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	593-599 bp	1.0	77.2
trnD-GUC-trnY-GUA_p5	CGGTGCTCTGACCAATTGAA	CGGACTGTAAATTCGTTGGC	50.0	50.0	58.5	57.5	589-595 bp	1.0	76.1