Hotspot

atpA-atpF

LSC · 11998-12084 · 87 bp

Back to Sterculia

Pi 0.045

MarkerSeek score 57.50

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 87
variable_sites: 5
pi: 0.045
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	GCCCACTTGAAGTACGGTAC	ATTGGCTTGTTTGGGGTGAT	55.0	45.0	58.3	58.0	221-234 bp	1.0	83.3
atpA-atpF_p2	CCCACTTGAAGTACGGTACC	ATTGGCTTGTTTGGGGTGAT	55.0	45.0	57.6	58.0	220-233 bp	1.0	82.6
atpA-atpF_p3	TTGCTCAATACGTTCACGGA	ATTGGCTTGTTTGGGGTGAT	45.0	45.0	57.6	58.0	170-183 bp	1.0	82.5
atpA-atpF_p4	TGCTCAATACGTTCACGGAT	ATTGGCTTGTTTGGGGTGAT	45.0	45.0	57.3	58.0	169-182 bp	1.0	80.8
atpA-atpF_p5	GCCCACTTGAAGTACGGTAC	GGCTTGTTTGGGGTGATGAA	55.0	50.0	58.3	58.7	218-231 bp	1.0	78.2