Hotspot

trnR-UCU-atpA

LSC · 10176-10473 · 298 bp

Back to Sterculia

Pi 0.078

MarkerSeek score 58.20

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 298
variable_sites: 24
pi: 0.078
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	ACGCTATTCAGGAACAAATGGA	47.6	40.9	57.3	58.0	277-370 bp	1.0	60.3
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	TGAAAGACGCTATTCAGGAACA	47.6	40.9	57.3	57.7	283-376 bp	1.0	58.4
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	GACGCTATTCAGGAACAAATGG	47.6	45.5	57.3	57.7	278-371 bp	1.0	58.4
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	ACGCTATTCAGGAACAAATGG	47.6	42.9	57.3	56.6	277-370 bp	1.0	57.5
trnR-UCU-atpA_p5	TGGATAGGACAGAGGTCTTCT	ACGCTATTCAGGAACAAATGGA	47.6	40.9	56.9	58.0	312-405 bp	1.0	56.8