Hotspot

trnK-UUU-rps16

LSC · 4312-5137 · 826 bp

Back to Sterculia

Pi 0.0262

MarkerSeek score 55.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 826
variable_sites: 32
pi: 0.0262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.2	58.0	954-1047 bp	1.0	82.1
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.2	58.0	953-1046 bp	1.0	82.1
trnK-UUU-rps16_p3	TACCGTTGAGTTAGCAACCC	AAAAGGAGCTCAACCCACAG	50.0	50.0	57.5	58.0	940-1033 bp	1.0	79.8
trnK-UUU-rps16_p4	AAAGCCGAGTACTCTACCGT	CGGGGGTTTTTACGGAACTT	50.0	50.0	58.2	58.4	907-1000 bp	1.0	79.2
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	CGGGGGTTTTTACGGAACTT	50.0	50.0	58.2	58.4	906-999 bp	1.0	79.2