Hotspot

ycf4

LSC · 60598-61143 · 546 bp

Back to Stephanodiscus

Pi 0.00977

MarkerSeek score 63.90

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 546
variable_sites: 8
pi: 0.00977
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	CCACTAAACTATGCCCGCAT	GGATTTTGCTTTGCACTTGC	50.0	45.0	58.0	57.0	683 bp	1.0	73.0
ycf4_p2	CGAACCCCCATCATTAGCTT	GGATTTTGCTTTGCACTTGC	50.0	45.0	57.7	57.0	720 bp	1.0	70.5
ycf4_p3	GGAATCGAACCCCCATCATT	GGATTTTGCTTTGCACTTGC	50.0	45.0	57.6	57.0	725 bp	1.0	70.3
ycf4_p4	ACCCCCATCATTAGCTTGGA	GGATTTTGCTTTGCACTTGC	50.0	45.0	58.4	57.0	717 bp	1.0	70.1
ycf4_p5	AACCCCCATCATTAGCTTGG	GGATTTTGCTTTGCACTTGC	50.0	45.0	57.5	57.0	718 bp	1.0	69.6