Hotspot

petB

LSC · 56844-57491 · 648 bp

Back to Stephanodiscus

Pi 0.0103

MarkerSeek score 65.30

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 648
variable_sites: 10
pi: 0.0103
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GATTCCGTGGGTTCGAATCC	TTATGACCCATGCCTTTAGC	55.0	45.0	58.7	55.5	880-881 bp	1.0	55.1
petB_p2	GGATTCCGTGGGTTCGAATC	TTATGACCCATGCCTTTAGC	55.0	45.0	58.7	55.5	881-882 bp	1.0	55.1
petB_p3	GAATCCCACTCTCGCCTTTA	TTATGACCCATGCCTTTAGC	50.0	45.0	57.0	55.5	866-867 bp	1.0	52.9
petB_p4	GATTCCGTGGGTTCGAATCC	ATTATGACCCATGCCTTTAGC	55.0	42.9	58.7	56.1	881-882 bp	1.0	51.7
petB_p5	GGATTCCGTGGGTTCGAATC	ATTATGACCCATGCCTTTAGC	55.0	42.9	58.7	56.1	882-883 bp	1.0	51.7