Hotspot

orf131

SSC · 93254-93649 · 396 bp

Back to Stephanodiscus

Pi 0.00842

MarkerSeek score 59.90

Primer available yes

Label orf131

Feature Metadata

label_name: orf131
feature_type: gene
region: SSC
length_bp: 396
variable_sites: 5
pi: 0.00842
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf131_p1	GGCGAGTTTGTTTCTACACG	CCGCACATATTTTGCTACTGC	50.0	47.6	57.4	58.3	589 bp	1.0	65.9
orf131_p2	GGCGAGTTTGTTTCTACACG	CGCACATATTTTGCTACTGCT	50.0	42.9	57.4	57.3	588 bp	1.0	62.7
orf131_p3	GGCGAGTTTGTTTCTACACG	CGCACATATTTTGCTACTGCTT	50.0	40.9	57.4	58.0	588 bp	1.0	59.9
orf131_p4	GGCGAGTTTGTTTCTACACG	CGCACATATTTTGCTACTGC	50.0	45.0	57.4	55.8	588 bp	1.0	58.7
orf131_p5	GCGAGTTTGTTTCTACACGA	CCGCACATATTTTGCTACTGC	45.0	47.6	56.4	58.3	588 bp	1.0	57.9