Hotspot

groEL-dnaK

SSC · 88236-88325 · 90 bp

Back to Stephanodiscus

Pi 0.0593

MarkerSeek score 71.30

Primer available yes

Label groEL-dnaK

Feature Metadata

label_name: groEL-dnaK
feature_type: igs
region: SSC
length_bp: 90
variable_sites: 8
pi: 0.0593
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
groEL-dnaK_p1	AAGACCGGATTGCCAAACTT	GTTGAAACTGTCGGTGGGAT	45.0	50.0	58.0	58.1	1208 bp	1.0	80.8
groEL-dnaK_p2	AGACCGGATTGCCAAACTTT	GTTGAAACTGTCGGTGGGAT	45.0	50.0	58.0	58.1	1207 bp	1.0	80.8
groEL-dnaK_p3	CTGTTTTAACTGGGGGCACT	GTTGAAACTGTCGGTGGGAT	50.0	50.0	58.0	58.1	1427 bp	1.0	80.5
groEL-dnaK_p4	AAGACCGGATTGCCAAACTT	TTGGTGTTGAAACTGTCGGT	45.0	45.0	58.0	57.9	1213 bp	0.666667	66.2
groEL-dnaK_p5	AGACCGGATTGCCAAACTTT	TTGGTGTTGAAACTGTCGGT	45.0	45.0	58.0	57.9	1212 bp	0.666667	66.2