Hotspot

thiS-psbW

SSC · 85312-85852 · 541 bp

Back to Stephanodiscus

Pi 0.0222

MarkerSeek score 62.60

Primer available yes

Label thiS-psbW

Feature Metadata

label_name: thiS-psbW
feature_type: igs
region: SSC
length_bp: 541
variable_sites: 18
pi: 0.0222
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
thiS-psbW_p1	TGAGATAGTTACTATTGTTGGGGG	AAGCTCTCAGGATTGGGACA	41.7	50.0	57.5	58.3	644-654 bp	1.0	44.8
thiS-psbW_p2	TGAGATAGTTACTATTGTTGGGGG	GCTCTCAGGATTGGGACAGA	41.7	55.0	57.5	58.8	642-652 bp	1.0	43.5
thiS-psbW_p3	TGAGATAGTTACTATTGTTGGGGG	AAAGCTCTCAGGATTGGGAC	41.7	50.0	57.5	57.2	645-655 bp	1.0	43.4
thiS-psbW_p4	TGAGATAGTTACTATTGTTGGGGG	AGCTCTCAGGATTGGGACAG	41.7	55.0	57.5	58.8	643-653 bp	1.0	43.4
thiS-psbW_p5	TTGAGATAGTTACTATTGTTGGGGG	AAGCTCTCAGGATTGGGACA	40.0	50.0	58.1	58.3	645-655 bp	1.0	43.4