Hotspot

ycf35-psaC

IRb · 82303-82394 · 92 bp

Back to Stephanodiscus

Pi 0.058

MarkerSeek score 71.30

Primer available yes

Label ycf35-psaC

Feature Metadata

label_name: ycf35-psaC
feature_type: igs
region: IRb
length_bp: 92
variable_sites: 8
pi: 0.058
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf35-psaC_p1	TGGAAGTAATGCACGAACGT	CTTCATCACCACGCGTAGAA	45.0	50.0	57.8	58.0	938 bp	1.0	80.2
ycf35-psaC_p2	TGGAAGTAATGCACGAACGT	TCACCACGCGTAGAAGATTG	45.0	50.0	57.8	58.0	933 bp	1.0	80.2
ycf35-psaC_p3	TGGAAGTAATGCACGAACGT	TTCATCACCACGCGTAGAAG	45.0	50.0	57.8	58.0	937 bp	1.0	80.2
ycf35-psaC_p4	TGGAAGTAATGCACGAACGT	ATTGTGTTGGGTGTAAGCGT	45.0	45.0	57.8	58.0	917 bp	1.0	80.1
ycf35-psaC_p5	TGGAAGTAATGCACGAACGT	TGGGTGTAAGCGTTGTGAAA	45.0	45.0	57.8	58.0	910 bp	1.0	80.0