Hotspot

cbbX-ycf39

LSC · 62804-62830 · 27 bp

Back to Stephanodiscus

Pi 0.0988

MarkerSeek score 81.50

Primer available yes

Label cbbX-ycf39

Feature Metadata

label_name: cbbX-ycf39
feature_type: igs
region: LSC
length_bp: 27
variable_sites: 4
pi: 0.0988
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cbbX-ycf39_p1	CGCCAAGCAAACCGTATTTT	TGACGTCCTAAAGTACCCGT	45.0	50.0	57.9	58.1	176 bp	1.0	80.6
cbbX-ycf39_p2	CGCCAAGCAAACCGTATTTT	CGTCCTAAAGTACCCGTTCC	45.0	55.0	57.9	57.7	173 bp	1.0	79.1
cbbX-ycf39_p3	CGCCAAGCAAACCGTATTTT	GTACCCGTTCCTCCAACAAT	45.0	50.0	57.9	57.5	164 bp	1.0	77.6
cbbX-ycf39_p4	CGCCAAGCAAACCGTATTTT	AAAGTACCCGTTCCTCCAAC	45.0	50.0	57.9	57.4	167 bp	1.0	77.0
cbbX-ycf39_p5	CGCCAAGCAAACCGTATTTT	AGTACCCGTTCCTCCAACAA	45.0	50.0	57.9	58.6	165 bp	1.0	76.9