Hotspot

rpoC1-rpoB

LSC · 34883-34891 · 9 bp

Back to Stephanodiscus

Pi 0.0741
MarkerSeek score 68.90
Primer available yes
Label rpoC1-rpoB

Feature Metadata

label_name
rpoC1-rpoB
feature_type
igs
region
LSC
length_bp
9
variable_sites
1
pi
0.0741
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1-rpoB_p1 AGGTAATTTTCTCTGGGACCA TGATGAGTTTAGTTCAGACGA 42.9 38.1 56.2 54.4 214 bp 1.0 40.4
rpoC1-rpoB_p2 TCTCTGGGACCATTGTAAGA TGATGAGTTTAGTTCAGACGA 45.0 38.1 55.2 54.4 205 bp 1.0 40.4
rpoC1-rpoB_p3 AGGTAATTTTCTCTGGGACCA TGATGAGTTTAGTTCAGACGAA 42.9 36.4 56.2 55.2 214 bp 1.0 40.4
rpoC1-rpoB_p4 AGGTAATTTTCTCTGGGACCA TTGATGAGTTTAGTTCAGACGA 42.9 36.4 56.2 55.2 215 bp 1.0 40.4
rpoC1-rpoB_p5 TTCTCTGGGACCATTGTAAGA TGATGAGTTTAGTTCAGACGA 42.9 38.1 56.0 54.4 206 bp 1.0 40.4