Hotspot

ycf45-trnF(gaa)

LSC · 22684-24060 · 1377 bp

Back to Stephanodiscus

Pi 0.0286

MarkerSeek score 68.30

Primer available yes

Label ycf45-trnF(gaa)

Feature Metadata

label_name: ycf45-trnF(gaa)
feature_type: igs
region: LSC
length_bp: 1377
variable_sites: 59
pi: 0.0286
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf45-trnF(gaa)_p1	TCAGAGAAGCATTTACCAAA	TCCACTGCTCTACCAACTGA	35.0	50.0	52.4	58.0	1458-1460 bp	1.0	43.3
ycf45-trnF(gaa)_p2	TCAGAGAAGCATTTACCAAA	TGGAGACACGAGGATCTTCA	35.0	50.0	52.4	57.8	1479-1481 bp	1.0	43.3
ycf45-trnF(gaa)_p3	TCAGAGAAGCATTTACCAAA	AACTGGAGACACGAGGATCT	35.0	50.0	52.4	57.8	1482-1484 bp	1.0	43.3
ycf45-trnF(gaa)_p4	TCAGAGAAGCATTTACCAAA	ACTGGAGACACGAGGATCTT	35.0	50.0	52.4	57.8	1481-1483 bp	1.0	43.3
ycf45-trnF(gaa)_p5	ATCAGAGAAGCATTTACCAA	TCCACTGCTCTACCAACTGA	35.0	50.0	52.1	58.0	1459-1461 bp	1.0	43.3