Hotspot

trnR(tct)-psaE

LSC · 19516-19692 · 177 bp

Back to Stephanodiscus

Pi 0.0343
MarkerSeek score 69.00
Primer available yes
Label trnR(tct)-psaE

Feature Metadata

label_name
trnR(tct)-psaE
feature_type
igs
region
LSC
length_bp
177
variable_sites
9
pi
0.0343
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR(tct)-psaE_p1 TCGTCTAACGGATCAGGACA TGATCCACTGTTGCAACTGT 50.0 45.0 57.9 57.7 323-329 bp 1.0 80.0
trnR(tct)-psaE_p2 TCGTCTAACGGATCAGGACA TCCACTGTTGCAACTGTTCC 50.0 50.0 57.9 58.6 320-326 bp 1.0 77.9
trnR(tct)-psaE_p3 TTCGATTCCTACTGGGCCTA TGATCCACTGTTGCAACTGT 50.0 45.0 57.5 57.7 277-283 bp 1.0 77.8
trnR(tct)-psaE_p4 CGTCTAACGGATCAGGACAG TGATCCACTGTTGCAACTGT 55.0 45.0 57.5 57.7 322-328 bp 1.0 77.1
trnR(tct)-psaE_p5 TCGTCTAACGGATCAGGACA CCACTTTGATCCACTGTTGC 50.0 50.0 57.9 57.3 329-335 bp 1.0 77.0