Hotspot

psbX-rbcS

LSC · 7557-7959 · 403 bp

Back to Stephanodiscus

Pi 0.0265

MarkerSeek score 66.90

Primer available yes

Label psbX-rbcS

Feature Metadata

label_name: psbX-rbcS
feature_type: igs
region: LSC
length_bp: 403
variable_sites: 16
pi: 0.0265
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbX-rbcS_p1	CCTGCTGTTAAACTCGCGAT	CCAGCTAACGAACCAGGTTT	50.0	50.0	58.6	58.1	561 bp	1.0	76.3
psbX-rbcS_p2	CCTGCTGTTAAACTCGCGAT	TTAGATCGTACAGAAGGCGC	50.0	50.0	58.6	57.5	537 bp	1.0	73.4
psbX-rbcS_p3	ACGCTCTACACGATCAGTTT	CCAGCTAACGAACCAGGTTT	45.0	50.0	57.0	58.1	628 bp	1.0	73.1
psbX-rbcS_p4	CCTGCTGTTAAACTCGCGAT	TAGATCGTACAGAAGGCGCA	50.0	50.0	58.6	58.6	536 bp	1.0	72.4
psbX-rbcS_p5	GTACGCTCTACACGATCAGT	CCAGCTAACGAACCAGGTTT	50.0	50.0	56.9	58.1	630 bp	1.0	72.3