primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score psbX-rbcS_p1 psbX-rbcS 1 CCTGCTGTTAAACTCGCGAT CCAGCTAACGAACCAGGTTT 20 20 50.0 50.0 58.6 58.1 10.4 6.2 0.747943 7557 7959 561-561 1.000000 76.3 psbX-rbcS_p2 psbX-rbcS 2 CCTGCTGTTAAACTCGCGAT TTAGATCGTACAGAAGGCGC 20 20 50.0 50.0 58.6 57.5 10.4 0.0 1.127104 7557 7959 537-537 1.000000 73.4 psbX-rbcS_p3 psbX-rbcS 3 ACGCTCTACACGATCAGTTT CCAGCTAACGAACCAGGTTT 20 20 45.0 50.0 57.0 58.1 0.0 6.2 1.119485 7557 7959 628-628 1.000000 73.1 psbX-rbcS_p4 psbX-rbcS 4 CCTGCTGTTAAACTCGCGAT TAGATCGTACAGAAGGCGCA 20 20 50.0 50.0 58.6 58.6 10.4 10.8 1.254624 7557 7959 536-536 1.000000 72.4 psbX-rbcS_p5 psbX-rbcS 5 GTACGCTCTACACGATCAGT CCAGCTAACGAACCAGGTTT 20 20 50.0 50.0 56.9 58.1 0.0 6.2 1.217862 7557 7959 630-630 1.000000 72.3 trnV(tac)-trnR(tct)_p1 trnV(tac)-trnR(tct) 1 AACTCAGCGGTAGAGTGTCT TGTCCTGATCCGTTAGACGA 20 20 50.0 50.0 58.1 57.9 16.6 1.5 0.205896 19430 19442 104-104 1.000000 79.1 trnV(tac)-trnR(tct)_p2 trnV(tac)-trnR(tct) 2 GAGTGTCTGCCTTACAAGCA TGTCCTGATCCGTTAGACGA 20 20 50.0 50.0 57.8 57.9 0.0 1.5 0.288146 19430 19442 92-92 1.000000 78.6 trnV(tac)-trnR(tct)_p3 trnV(tac)-trnR(tct) 3 GCCTTACAAGCAGAAGGTCA TGTCCTGATCCGTTAGACGA 20 20 50.0 50.0 57.8 57.9 2.8 1.5 0.303317 19430 19442 84-84 1.000000 78.5 trnV(tac)-trnR(tct)_p4 trnV(tac)-trnR(tct) 4 TGCCTTACAAGCAGAAGGTC TGTCCTGATCCGTTAGACGA 20 20 50.0 50.0 57.8 57.9 2.8 1.5 0.303317 19430 19442 85-85 1.000000 78.5 trnV(tac)-trnR(tct)_p5 trnV(tac)-trnR(tct) 5 CAAGCAGAAGGTCACAGGTT TAGGCCCAGTAGGAATCGAA 20 20 50.0 50.0 58.0 57.5 0.0 0.0 0.491923 19430 19442 124-124 1.000000 76.7 trnR(tct)-psaE_p1 trnR(tct)-psaE 1 TCGTCTAACGGATCAGGACA TGATCCACTGTTGCAACTGT 20 20 50.0 45.0 57.9 57.7 0.0 29.8 0.465080 19516 19692 323-329 1.000000 80.0 trnR(tct)-psaE_p2 trnR(tct)-psaE 2 TCGTCTAACGGATCAGGACA TCCACTGTTGCAACTGTTCC 20 20 50.0 50.0 57.9 58.6 0.0 32.0 0.729766 19516 19692 320-326 1.000000 77.9 trnR(tct)-psaE_p3 trnR(tct)-psaE 3 TTCGATTCCTACTGGGCCTA TGATCCACTGTTGCAACTGT 20 20 50.0 45.0 57.5 57.7 4.5 29.8 0.811562 19516 19692 277-283 1.000000 77.8 trnR(tct)-psaE_p4 trnR(tct)-psaE 4 CGTCTAACGGATCAGGACAG TGATCCACTGTTGCAACTGT 20 20 55.0 45.0 57.5 57.7 0.0 29.8 0.823639 19516 19692 322-328 1.000000 77.1 trnR(tct)-psaE_p5 trnR(tct)-psaE 5 TCGTCTAACGGATCAGGACA CCACTTTGATCCACTGTTGC 20 20 50.0 50.0 57.9 57.3 0.0 0.0 0.830370 19516 19692 329-335 1.000000 77.0 psaE_p1 psaE 1 CAAGCAGAAGGTCACAGGTT TCCAATTTGCAGGACAAGCT 20 20 50.0 45.0 58.0 58.0 0.0 0.0 0.027480 19693 19890 1173-1180 1.000000 81.6 psaE_p2 psaE 2 GCAGAAGGTCACAGGTTCAA TCCAATTTGCAGGACAAGCT 20 20 50.0 45.0 58.0 58.0 0.0 0.0 0.033033 19693 19890 1170-1177 1.000000 81.6 psaE_p3 psaE 3 AACTCAGCGGTAGAGTGTCT TCCAATTTGCAGGACAAGCT 20 20 50.0 45.0 58.1 58.0 16.6 0.0 0.087935 19693 19890 1199-1206 1.000000 81.1 psaE_p4 psaE 4 TACGCCCGTTAAATGCTGAA TCCAATTTGCAGGACAAGCT 20 20 45.0 45.0 57.9 58.0 0.0 0.0 0.096572 19693 19890 1552-1559 1.000000 80.9 psaE_p5 psaE 5 TTACGCCCGTTAAATGCTGA TCCAATTTGCAGGACAAGCT 20 20 45.0 45.0 57.9 58.0 0.0 0.0 0.096572 19693 19890 1553-1560 1.000000 80.9 ycf45-trnF(gaa)_p1 ycf45-trnF(gaa) 1 TCAGAGAAGCATTTACCAAA TCCACTGCTCTACCAACTGA 20 20 35.0 50.0 52.4 58.0 0.0 0.0 5.623205 22684 24060 1458-1460 1.000000 43.3 ycf45-trnF(gaa)_p2 ycf45-trnF(gaa) 2 TCAGAGAAGCATTTACCAAA TGGAGACACGAGGATCTTCA 20 20 35.0 50.0 52.4 57.8 0.0 5.6 5.829466 22684 24060 1479-1481 1.000000 43.3 ycf45-trnF(gaa)_p3 ycf45-trnF(gaa) 3 TCAGAGAAGCATTTACCAAA AACTGGAGACACGAGGATCT 20 20 35.0 50.0 52.4 57.8 0.0 0.0 5.837327 22684 24060 1482-1484 1.000000 43.3 ycf45-trnF(gaa)_p4 ycf45-trnF(gaa) 4 TCAGAGAAGCATTTACCAAA ACTGGAGACACGAGGATCTT 20 20 35.0 50.0 52.4 57.8 0.0 0.0 5.837327 22684 24060 1481-1483 1.000000 43.3 ycf45-trnF(gaa)_p5 ycf45-trnF(gaa) 5 ATCAGAGAAGCATTTACCAA TCCACTGCTCTACCAACTGA 20 20 35.0 50.0 52.1 58.0 0.0 0.0 5.873332 22684 24060 1459-1461 1.000000 43.3 rpoC1-rpoB_p1 rpoC1-rpoB 1 AGGTAATTTTCTCTGGGACCA TGATGAGTTTAGTTCAGACGA 21 21 42.9 38.1 56.2 54.4 0.0 0.0 7.384652 34883 34891 214-214 1.000000 40.4 rpoC1-rpoB_p2 rpoC1-rpoB 2 TCTCTGGGACCATTGTAAGA TGATGAGTTTAGTTCAGACGA 20 21 45.0 38.1 55.2 54.4 0.0 0.0 7.472069 34883 34891 205-205 1.000000 40.4 rpoC1-rpoB_p3 rpoC1-rpoB 3 AGGTAATTTTCTCTGGGACCA TGATGAGTTTAGTTCAGACGAA 21 22 42.9 36.4 56.2 55.2 0.0 0.0 7.577176 34883 34891 214-214 1.000000 40.4 rpoC1-rpoB_p4 rpoC1-rpoB 4 AGGTAATTTTCTCTGGGACCA TTGATGAGTTTAGTTCAGACGA 21 22 42.9 36.4 56.2 55.2 0.0 0.0 7.577176 34883 34891 215-215 1.000000 40.4 rpoC1-rpoB_p5 rpoC1-rpoB 5 TTCTCTGGGACCATTGTAAGA TGATGAGTTTAGTTCAGACGA 21 21 42.9 38.1 56.0 54.4 0.0 0.0 7.642677 34883 34891 206-206 1.000000 40.4 petA-chlI_p1 petA-chlI 1 AGCTCAGCACGAATTATAGGA CGTCACCGTTTACGAAAAGA 21 20 42.9 45.0 56.6 56.4 0.0 3.5 3.944476 44930 45443 726-727 1.000000 50.6 petA-chlI_p2 petA-chlI 2 TGAAAAAGTTCAAGCTGCTGA CGTCACCGTTTACGAAAAGA 21 20 38.1 45.0 56.6 56.4 1.4 3.5 3.985820 44930 45443 640-641 1.000000 50.6 petA-chlI_p3 petA-chlI 3 TGAAAAAGTTCAAGCTGCTGAA CGTCACCGTTTACGAAAAGA 22 20 36.4 45.0 57.3 56.4 1.4 3.5 4.278661 44930 45443 640-641 1.000000 48.3 petA-chlI_p4 petA-chlI 4 AGCTCAGCACGAATTATAGGA TCACCGTTTACGAAAAGATCCA 21 22 42.9 40.9 56.6 58.0 0.0 3.5 4.390405 44930 45443 724-725 1.000000 47.1 petA-chlI_p5 petA-chlI 5 AGCTCAGCACGAATTATAGGA CGTCACCGTTTACGAAAAGATC 21 22 42.9 45.5 56.6 58.0 0.0 3.5 4.412823 44930 45443 726-727 1.000000 46.9 ftsH-trnT(tgt)_p1 ftsH-trnT(tgt) 1 GCTCGTAAAATTTGTTGTCCCA AACCCCCAACCTACGGATTA 22 20 40.9 50.0 57.8 58.0 0.0 0.0 2.262005 49220 49485 343-343 1.000000 64.5 ftsH-trnT(tgt)_p2 ftsH-trnT(tgt) 2 TGCTCGTAAAATTTGTTGTCCC AACCCCCAACCTACGGATTA 22 20 40.9 50.0 57.8 58.0 0.0 0.0 2.262005 49220 49485 344-344 1.000000 64.5 ftsH-trnT(tgt)_p3 ftsH-trnT(tgt) 3 GCTCGTAAAATTTGTTGTCCCA TCTACCGTTGAGCTACACCA 22 20 40.9 50.0 57.8 58.1 0.0 0.0 2.317940 49220 49485 311-311 1.000000 64.3 ftsH-trnT(tgt)_p4 ftsH-trnT(tgt) 4 TGCTCGTAAAATTTGTTGTCCC TCTACCGTTGAGCTACACCA 22 20 40.9 50.0 57.8 58.1 0.0 0.0 2.317940 49220 49485 312-312 1.000000 64.3 ftsH-trnT(tgt)_p5 ftsH-trnT(tgt) 5 GCTCGTAAAATTTGTTGTCCCA ATTTGAACCCCCAACCTACG 22 20 40.9 50.0 57.8 57.8 0.0 0.0 2.441408 49220 49485 348-348 1.000000 63.0 petB_p1 petB 1 GATTCCGTGGGTTCGAATCC TTATGACCCATGCCTTTAGC 20 20 55.0 45.0 58.7 55.5 21.1 0.0 3.227211 56844 57491 880-881 1.000000 55.1 petB_p2 petB 2 GGATTCCGTGGGTTCGAATC TTATGACCCATGCCTTTAGC 20 20 55.0 45.0 58.7 55.5 0.0 0.0 3.227211 56844 57491 881-882 1.000000 55.1 petB_p3 petB 3 GAATCCCACTCTCGCCTTTA TTATGACCCATGCCTTTAGC 20 20 50.0 45.0 57.0 55.5 0.0 0.0 3.505797 56844 57491 866-867 1.000000 52.9 petB_p4 petB 4 GATTCCGTGGGTTCGAATCC ATTATGACCCATGCCTTTAGC 20 21 55.0 42.9 58.7 56.1 21.1 0.0 3.651677 56844 57491 881-882 1.000000 51.7 petB_p5 petB 5 GGATTCCGTGGGTTCGAATC ATTATGACCCATGCCTTTAGC 20 21 55.0 42.9 58.7 56.1 0.0 0.0 3.651677 56844 57491 882-883 1.000000 51.7 ycf4_p1 ycf4 1 CCACTAAACTATGCCCGCAT GGATTTTGCTTTGCACTTGC 20 20 50.0 45.0 58.0 57.0 7.4 0.0 0.988288 60598 61143 683-683 1.000000 73.0 ycf4_p2 ycf4 2 CGAACCCCCATCATTAGCTT GGATTTTGCTTTGCACTTGC 20 20 50.0 45.0 57.7 57.0 0.0 0.0 1.304017 60598 61143 720-720 1.000000 70.5 ycf4_p3 ycf4 3 GGAATCGAACCCCCATCATT GGATTTTGCTTTGCACTTGC 20 20 50.0 45.0 57.6 57.0 0.0 0.0 1.320579 60598 61143 725-725 1.000000 70.3 ycf4_p4 ycf4 4 ACCCCCATCATTAGCTTGGA GGATTTTGCTTTGCACTTGC 20 20 50.0 45.0 58.4 57.0 2.8 0.0 1.348382 60598 61143 717-717 1.000000 70.1 ycf4_p5 ycf4 5 AACCCCCATCATTAGCTTGG GGATTTTGCTTTGCACTTGC 20 20 50.0 45.0 57.5 57.0 0.0 0.0 1.415162 60598 61143 718-718 1.000000 69.6 cbbX-ycf39_p1 cbbX-ycf39 1 CGCCAAGCAAACCGTATTTT TGACGTCCTAAAGTACCCGT 20 20 45.0 50.0 57.9 58.1 0.0 14.1 0.149214 62804 62830 176-176 1.000000 80.6 cbbX-ycf39_p2 cbbX-ycf39 2 CGCCAAGCAAACCGTATTTT CGTCCTAAAGTACCCGTTCC 20 20 45.0 55.0 57.9 57.7 0.0 0.0 0.343176 62804 62830 173-173 1.000000 79.1 cbbX-ycf39_p3 cbbX-ycf39 3 CGCCAAGCAAACCGTATTTT GTACCCGTTCCTCCAACAAT 20 20 45.0 50.0 57.9 57.5 0.0 0.0 0.543656 62804 62830 164-164 1.000000 77.6 cbbX-ycf39_p4 cbbX-ycf39 4 CGCCAAGCAAACCGTATTTT AAAGTACCCGTTCCTCCAAC 20 20 45.0 50.0 57.9 57.4 0.0 0.0 0.615057 62804 62830 167-167 1.000000 77.0 cbbX-ycf39_p5 cbbX-ycf39 5 CGCCAAGCAAACCGTATTTT AGTACCCGTTCCTCCAACAA 20 20 45.0 50.0 57.9 58.6 0.0 0.0 0.632163 62804 62830 165-165 1.000000 76.9 ycf35-psaC_p1 ycf35-psaC 1 TGGAAGTAATGCACGAACGT CTTCATCACCACGCGTAGAA 20 20 45.0 50.0 57.8 58.0 0.0 13.4 0.167035 82303 82394 938-938 1.000000 80.2 ycf35-psaC_p2 ycf35-psaC 2 TGGAAGTAATGCACGAACGT TCACCACGCGTAGAAGATTG 20 20 45.0 50.0 57.8 58.0 0.0 13.4 0.167035 82303 82394 933-933 1.000000 80.2 ycf35-psaC_p3 ycf35-psaC 3 TGGAAGTAATGCACGAACGT TTCATCACCACGCGTAGAAG 20 20 45.0 50.0 57.8 58.0 0.0 13.4 0.167035 82303 82394 937-937 1.000000 80.2 ycf35-psaC_p4 ycf35-psaC 4 TGGAAGTAATGCACGAACGT ATTGTGTTGGGTGTAAGCGT 20 20 45.0 45.0 57.8 58.0 0.0 0.0 0.182175 82303 82394 917-917 1.000000 80.1 ycf35-psaC_p5 ycf35-psaC 5 TGGAAGTAATGCACGAACGT TGGGTGTAAGCGTTGTGAAA 20 20 45.0 45.0 57.8 58.0 0.0 0.0 0.197652 82303 82394 910-910 1.000000 80.0 thiS-psbW_p1 thiS-psbW 1 TGAGATAGTTACTATTGTTGGGGG AAGCTCTCAGGATTGGGACA 24 20 41.7 50.0 57.5 58.3 6.3 0.0 4.833203 85312 85852 644-654 1.000000 44.8 thiS-psbW_p2 thiS-psbW 2 TGAGATAGTTACTATTGTTGGGGG GCTCTCAGGATTGGGACAGA 24 20 41.7 55.0 57.5 58.8 6.3 0.0 5.295953 85312 85852 642-652 1.000000 43.5 thiS-psbW_p3 thiS-psbW 3 TGAGATAGTTACTATTGTTGGGGG AAAGCTCTCAGGATTGGGAC 24 20 41.7 50.0 57.5 57.2 6.3 0.0 5.291843 85312 85852 645-655 1.000000 43.4 thiS-psbW_p4 thiS-psbW 4 TGAGATAGTTACTATTGTTGGGGG AGCTCTCAGGATTGGGACAG 24 20 41.7 55.0 57.5 58.8 6.3 0.0 5.292336 85312 85852 643-653 1.000000 43.4 thiS-psbW_p5 thiS-psbW 5 TTGAGATAGTTACTATTGTTGGGGG AAGCTCTCAGGATTGGGACA 25 20 40.0 50.0 58.1 58.3 6.3 0.0 5.442755 85312 85852 645-655 1.000000 43.4 groEL-dnaK_p1 groEL-dnaK 1 AAGACCGGATTGCCAAACTT GTTGAAACTGTCGGTGGGAT 20 20 45.0 50.0 58.0 58.1 9.5 0.0 0.112414 88236 88325 1208-1208 1.000000 80.8 groEL-dnaK_p2 groEL-dnaK 2 AGACCGGATTGCCAAACTTT GTTGAAACTGTCGGTGGGAT 20 20 45.0 50.0 58.0 58.1 9.5 0.0 0.112414 88236 88325 1207-1207 1.000000 80.8 groEL-dnaK_p3 groEL-dnaK 3 CTGTTTTAACTGGGGGCACT GTTGAAACTGTCGGTGGGAT 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.112414 88236 88325 1427-1427 1.000000 80.5 groEL-dnaK_p4 groEL-dnaK 4 AAGACCGGATTGCCAAACTT TTGGTGTTGAAACTGTCGGT 20 20 45.0 45.0 58.0 57.9 9.5 0.0 0.121451 88236 88325 1213-1213 0.666667 66.2 groEL-dnaK_p5 groEL-dnaK 5 AGACCGGATTGCCAAACTTT TTGGTGTTGAAACTGTCGGT 20 20 45.0 45.0 58.0 57.9 9.5 0.0 0.121451 88236 88325 1212-1212 0.666667 66.2 dnaK_p1 dnaK 1 AAGACCGGATTGCCAAACTT CAGCAGGATCATTCACACGA 20 20 45.0 50.0 58.0 58.0 9.5 0.0 0.018959 88326 90122 2987-2987 1.000000 81.4 dnaK_p2 dnaK 2 AAGACCGGATTGCCAAACTT TCAGCAGGATCATTCACACG 20 20 45.0 50.0 58.0 58.0 9.5 0.0 0.018959 88326 90122 2988-2988 1.000000 81.4 dnaK_p3 dnaK 3 AGACCGGATTGCCAAACTTT CAGCAGGATCATTCACACGA 20 20 45.0 50.0 58.0 58.0 9.5 0.0 0.018959 88326 90122 2986-2986 1.000000 81.4 dnaK_p4 dnaK 4 AGACCGGATTGCCAAACTTT TCAGCAGGATCATTCACACG 20 20 45.0 50.0 58.0 58.0 9.5 0.0 0.018959 88326 90122 2987-2987 1.000000 81.4 dnaK_p5 dnaK 5 CTTTCAGGGGGAATTGCTGT CAGCAGGATCATTCACACGA 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.085467 88326 90122 2970-2970 1.000000 80.9 rpl3_p1 rpl3 1 CGTTTAGCAAGTTGGCCAAC TTTACCTCCTCCACGAACCT 20 20 50.0 50.0 58.2 58.0 36.6 0.0 0.253738 90449 91072 1365-1365 1.000000 79.6 rpl3_p2 rpl3 2 GTTGTTCGAAAACCTTCCGC TTTACCTCCTCCACGAACCT 20 20 50.0 50.0 58.2 58.0 0.0 0.0 0.269456 90449 91072 1305-1305 1.000000 79.5 rpl3_p3 rpl3 3 CGTTTAGCAAGTTGGCCAAC AATGGAGAACGGCTTGATCC 20 20 50.0 50.0 58.2 58.0 36.6 0.0 0.278054 90449 91072 1424-1424 1.000000 79.3 rpl3_p4 rpl3 4 GTTGTTCGAAAACCTTCCGC AATGGAGAACGGCTTGATCC 20 20 50.0 50.0 58.2 58.0 0.0 0.0 0.293771 90449 91072 1364-1364 1.000000 79.3 rpl3_p5 rpl3 5 CGTTTAGCAAGTTGGCCAAC CCCCACCTCTCCATAATGGA 20 20 50.0 55.0 58.2 58.2 36.6 0.0 0.414178 90449 91072 1438-1438 1.000000 78.2 orf131_p1 orf131 1 GGCGAGTTTGTTTCTACACG CCGCACATATTTTGCTACTGC 20 21 50.0 47.6 57.4 58.3 0.0 4.9 1.852376 93254 93649 589-589 1.000000 65.9 orf131_p2 orf131 2 GGCGAGTTTGTTTCTACACG CGCACATATTTTGCTACTGCT 20 21 50.0 42.9 57.4 57.3 0.0 4.9 2.252815 93254 93649 588-588 1.000000 62.7 orf131_p3 orf131 3 GGCGAGTTTGTTTCTACACG CGCACATATTTTGCTACTGCTT 20 22 50.0 40.9 57.4 58.0 0.0 4.9 2.592092 93254 93649 588-588 1.000000 59.9 orf131_p4 orf131 4 GGCGAGTTTGTTTCTACACG CGCACATATTTTGCTACTGC 20 20 50.0 45.0 57.4 55.8 0.0 4.9 2.746169 93254 93649 588-588 1.000000 58.7 orf131_p5 orf131 5 GCGAGTTTGTTTCTACACGA CCGCACATATTTTGCTACTGC 20 21 45.0 47.6 56.4 58.3 0.0 4.9 2.850992 93254 93649 588-588 1.000000 57.9 psaC-ycf35_p1 psaC-ycf35 1 CTTCATCACCACGCGTAGAA TGGAAGTAATGCACGAACGT 20 20 50.0 45.0 58.0 57.8 13.4 0.0 0.167035 112497 112588 938-938 1.000000 80.2 psaC-ycf35_p2 psaC-ycf35 2 TCACCACGCGTAGAAGATTG TGGAAGTAATGCACGAACGT 20 20 50.0 45.0 58.0 57.8 13.4 0.0 0.167035 112497 112588 933-933 1.000000 80.2 psaC-ycf35_p3 psaC-ycf35 3 TTCATCACCACGCGTAGAAG TGGAAGTAATGCACGAACGT 20 20 50.0 45.0 58.0 57.8 13.4 0.0 0.167035 112497 112588 937-937 1.000000 80.2 psaC-ycf35_p4 psaC-ycf35 4 ATTGTGTTGGGTGTAAGCGT TGGAAGTAATGCACGAACGT 20 20 45.0 45.0 58.0 57.8 0.0 0.0 0.182175 112497 112588 917-917 1.000000 80.1 psaC-ycf35_p5 psaC-ycf35 5 TGGGTGTAAGCGTTGTGAAA TGGAAGTAATGCACGAACGT 20 20 45.0 45.0 58.0 57.8 0.0 0.0 0.197652 112497 112588 910-910 1.000000 80.0