Hotspot

ycf1

SSC · 127767-133355 · 5589 bp

Back to Stephania

Pi 0.0224

MarkerSeek score 57.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5589
variable_sites: 411
pi: 0.0224
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCACGATAGGATCCGTTCA	AGAATGCCGTCGCCTATTTT	50.0	45.0	57.9	58.0	699-789 bp	1.0	89.8
ycf1_p2	TCCACGATAGGATCCGTTCA	GAGGTATCAGCAACGACTGG	50.0	55.0	57.9	58.1	1212-1302 bp	1.0	89.5
ycf1_p3	TCCACGATAGGATCCGTTCA	GGAGGTATCAGCAACGACTG	50.0	55.0	57.9	58.1	1213-1303 bp	1.0	89.5
ycf1_p4	TCCACGATAGGATCCGTTCA	GCCGTCGCCTATTTTCACTA	50.0	50.0	57.9	58.1	694-784 bp	0.952381	87.6
ycf1_p5	TCCACGATAGGATCCGTTCA	AAGAATGCCGTCGCCTATTT	50.0	45.0	57.9	58.0	700-790 bp	0.666667	75.3