Hotspot

psaC-ndhE

SSC · 120917-121206 · 290 bp

Back to Stephania

Pi 0.032

MarkerSeek score 70.10

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 290
variable_sites: 28
pi: 0.032
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACACCCATCCCAAGGTATCA	GCTATTGCAGCCGCTGAA	50.0	55.6	58.0	58.5	467-514 bp	1.0	69.0
psaC-ndhE_p2	ACACCCATCCCAAGGTATCA	TATTGCAGCCGCTGAAGC	50.0	55.6	58.0	58.5	465-512 bp	1.0	69.0
psaC-ndhE_p3	CTAATACATCCGTGGGGCAG	TATTGCAGCCGCTGAAGC	55.0	55.6	57.8	58.5	442-489 bp	1.0	68.0
psaC-ndhE_p4	ACACCCATCCCAAGGTATCA	CTATTGCAGCCGCTGAAGC	50.0	57.9	58.0	59.6	466-513 bp	1.0	67.8
psaC-ndhE_p5	ACACCCATCCCAAGGTATCA	GCTATTGCAGCCGCTGAAG	50.0	57.9	58.0	59.6	467-514 bp	1.0	67.8