Hotspot

ccsA-ndhD

SSC · 118747-119044 · 298 bp

Back to Stephania

Pi 0.0459

MarkerSeek score 74.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 298
variable_sites: 36
pi: 0.0459
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GAGCGGTATGGGCTAATGAG	TTTTTGGATTCTGGACCCCG	55.0	50.0	57.9	58.1	655-691 bp	1.0	88.8
ccsA-ndhD_p2	GAGCGGTATGGGCTAATGAG	TTCTCGATGGCTTCTCTTGC	55.0	50.0	57.9	58.0	874-910 bp	1.0	87.8
ccsA-ndhD_p3	GAGCGGTATGGGCTAATGAG	GGGAATGAGCGGTTTTGTTG	55.0	50.0	57.9	57.9	848-884 bp	1.0	87.5
ccsA-ndhD_p4	GAGCGGTATGGGCTAATGAG	TTCAACATCTCCTGGCCAAC	55.0	50.0	57.9	58.1	1139-1175 bp	1.0	86.0
ccsA-ndhD_p5	GAGCGGTATGGGCTAATGAG	GTTGGTGATAGTGGGCACAA	55.0	50.0	57.9	58.1	1178-1214 bp	1.0	85.7