Hotspot

ndhF-rpl32

SSC · 115347-116664 · 1318 bp

Back to Stephania

Pi 0.0373
MarkerSeek score 75.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1318
variable_sites
160
pi
0.0373
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.98
markerseek_score
75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAACGAAAGGT TGCGACAGTAAAGCGATCAA 50.0 45.0 57.7 57.9 1940-2003 bp 1.0 89.4
ndhF-rpl32_p2 AACAAGGAGAAGCCCCAATC TGCGACAGTAAAGCGATCAA 50.0 45.0 57.8 57.9 1975-2007 bp 0.714286 74.2
ndhF-rpl32_p3 AACAAGGAGAAGCCCCAATC TTGCGACAGTAAAGCGATCA 50.0 45.0 57.8 57.9 1976-2008 bp 0.714286 74.2
ndhF-rpl32_p4 GAACAAGGAGAAGCCCCAAT TGCGACAGTAAAGCGATCAA 50.0 45.0 57.8 57.9 1976-2008 bp 0.714286 74.2
ndhF-rpl32_p5 GAACAAGGAGAAGCCCCAAT TTGCGACAGTAAAGCGATCA 50.0 45.0 57.8 57.9 1977-2009 bp 0.714286 74.2