Hotspot

trnP-UGG-psaJ

LSC · 70174-70889 · 716 bp

Back to Stephania

Pi 0.0358

MarkerSeek score 80.40

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 79
pi: 0.0358
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	ACTTAACACAGGTGCCACAG	50.0	50.0	58.1	58.0	816-893 bp	1.0	88.1
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	ACTTAACACAGGTGCCACAG	50.0	50.0	58.1	58.0	815-892 bp	1.0	88.1
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	ACTTAACACAGGTGCCACAG	50.0	50.0	58.1	58.0	827-904 bp	1.0	87.9
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	ACTTAACACAGGTGCCACAG	50.0	50.0	58.1	58.0	826-903 bp	1.0	87.9
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	ACTTAACACAGGTGCCACAG	50.0	50.0	58.1	58.0	829-906 bp	1.0	87.9