Hotspot

accD-psaI

LSC · 62400-63124 · 725 bp

Back to Stephania

Pi 0.0264

MarkerSeek score 71.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 725
variable_sites: 68
pi: 0.0264
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AATGCCTACATTGCGTTTGC	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	886-1016 bp	1.0	89.9
accD-psaI_p2	AATGCCTACATTGCGTTTGC	TGTTTTTCGAGACCCCGTTT	45.0	45.0	58.0	58.0	1393-1518 bp	1.0	89.0
accD-psaI_p3	AATGCCTACATTGCGTTTGC	AATGAACAGACCTGCGATCC	45.0	50.0	58.0	58.0	1564-1689 bp	1.0	88.6
accD-psaI_p4	AATGCCTACATTGCGTTTGC	GCTAATGAACAGACCTGCGA	45.0	50.0	58.0	58.0	1567-1692 bp	0.714286	75.4
accD-psaI_p5	AATGCCTACATTGCGTTTGC	AGCTAATGAACAGACCTGCG	45.0	50.0	58.0	58.0	1568-1693 bp	0.714286	75.4