Hotspot

trnT-GGU-psbD

LSC · 34197-35723 · 1527 bp

Back to Stephania

Pi 0.0204

MarkerSeek score 69.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1527
variable_sites: 105
pi: 0.0204
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1690-1836 bp	1.0	86.0
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	GACCGGACCAACCTACAAAA	50.0	50.0	57.9	57.7	1704-1850 bp	1.0	85.9
trnT-GGU-psbD_p3	ATGGGCCCTTTTAACTCAGC	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1694-1840 bp	1.0	85.7
trnT-GGU-psbD_p4	ATGGGCCCTTTTAACTCAGC	GACCGGACCAACCTACAAAA	50.0	50.0	58.1	57.7	1708-1854 bp	1.0	85.5
trnT-GGU-psbD_p5	GCCCTTTTAACTCAGCGGTA	TAGACCGGACCAACCTACAA	50.0	50.0	57.9	57.7	1706-1852 bp	1.0	85.5