Hotspot

psbI-trnS-GCU

LSC · 8600-8741 · 142 bp

Back to Stephania

Pi 0.0753

MarkerSeek score 72.50

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 142
variable_sites: 32
pi: 0.0753
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	AATCCTGGGCGTGAGGAATA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	58.5	58.0	231-238 bp	1.0	87.8
psbI-trnS-GCU_p2	ATCCTGGGCGTGAGGAATAA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	58.5	58.0	230-237 bp	1.0	87.8
psbI-trnS-GCU_p3	TAATCCTGGGCGTGAGGAAT	TAAAGCGGCGGATTGCTAAT	50.0	45.0	58.5	58.0	232-239 bp	1.0	87.8
psbI-trnS-GCU_p4	AATCCTGGGCGTGAGGAATA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	58.5	58.1	255-262 bp	1.0	86.9
psbI-trnS-GCU_p5	ATCCTGGGCGTGAGGAATAA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	58.5	58.1	254-261 bp	1.0	86.9