Hotspot

psbK-psbI

LSC · 8069-8488 · 420 bp

Back to Stephania

Pi 0.0327

MarkerSeek score 79.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 420
variable_sites: 45
pi: 0.0327
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TTTTATTCCTCACGCCCAGG	50.0	50.0	57.9	57.9	450-602 bp	1.0	89.2
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	TTTTATTCCTCACGCCCAGG	45.0	50.0	58.3	57.9	447-599 bp	1.0	87.2
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	TTTTATTCCTCACGCCCAGG	45.0	50.0	58.3	57.9	448-600 bp	1.0	87.2
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	TTTTATTCCTCACGCCCAGG	45.0	50.0	58.3	57.9	449-601 bp	1.0	87.2
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	ATTCCTCACGCCCAGGATTA	50.0	50.0	57.9	58.5	446-598 bp	1.0	86.3