Hotspot

trnK-UUU-rps16

LSC · 4666-5704 · 1039 bp

Back to Stephania

Pi 0.0366

MarkerSeek score 82.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1039
variable_sites: 121
pi: 0.0366
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTACGGACGAATCCATTGC	CCATCTACCGAATCGTTGCA	50.0	50.0	58.1	58.1	1078-1233 bp	1.0	91.0
trnK-UUU-rps16_p2	GTACGGACGAATCCATTGCT	CCATCTACCGAATCGTTGCA	50.0	50.0	58.1	58.1	1077-1232 bp	1.0	91.0
trnK-UUU-rps16_p3	AGTACGGACGAATCCATTGC	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.1	57.9	1050-1205 bp	1.0	90.4
trnK-UUU-rps16_p4	GTACGGACGAATCCATTGCT	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.1	57.9	1049-1204 bp	1.0	90.4
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	CCATCTACCGAATCGTTGCA	50.0	50.0	58.2	58.1	1021-1176 bp	1.0	90.1