Hotspot

trnH-GUG-psbA

LSC · 77-603 · 527 bp

Back to Stephania

Pi 0.0375

MarkerSeek score 73.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 527
variable_sites: 83
pi: 0.0375
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	AATCCACTTGGCTACATCCG	TGCTCACAACTTCCCTCTAGA	50.0	47.6	57.7	58.1	598-674 bp	1.0	80.5
trnH-GUG-psbA_p2	AATCCACTTGGCTACATCCG	CCCTCTAGATCTAGCTGCTGT	50.0	52.4	57.7	58.1	586-662 bp	1.0	80.3
trnH-GUG-psbA_p3	AATCCACTTGGCTACATCCG	TCCCTCTAGATCTAGCTGCTG	50.0	52.4	57.7	57.8	587-663 bp	1.0	80.1
trnH-GUG-psbA_p4	AATCCACTTGGCTACATCCG	GCATGAACGTAATGCTCACA	50.0	45.0	57.7	56.8	610-686 bp	1.0	79.9
trnH-GUG-psbA_p5	AATCCACTTGGCTACATCCG	CGTAATGCTCACAACTTCCC	50.0	50.0	57.7	56.8	603-679 bp	1.0	79.9