Hotspot

infA-rps8

LSC · 77971-78051 · 81 bp

Back to Stenotaphrum

Pi 0.0494

MarkerSeek score 67.40

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 81
variable_sites: 4
pi: 0.0494
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	CGCAAACCAATACGCACTTT	TTAAAGCGTATCAGCCGACC	45.0	50.0	58.0	58.1	1000-1001 bp	1.0	80.7
infA-rps8_p2	GCCCCTTGCTGGAATCATAA	TTAAAGCGTATCAGCCGACC	50.0	50.0	57.9	58.1	463-464 bp	1.0	80.4
infA-rps8_p3	AATTATACGCCCCTTGCTGG	TTAAAGCGTATCAGCCGACC	50.0	50.0	58.0	58.1	471 bp	0.5	59.3
infA-rps8_p4	AATTATACGCCCCTTGCTGG	GCGATTGCCGAAGAATTGAG	50.0	50.0	58.0	57.9	919 bp	0.5	59.1
infA-rps8_p5	GTGGTGTCATTCTCTAGGCG	TTAAAGCGTATCAGCCGACC	55.0	50.0	58.1	58.1	359 bp	0.5	58.9