Hotspot

psbT-psbN

LSC · 72076-72149 · 74 bp

Back to Stenotaphrum

Pi 0.0405

MarkerSeek score 75.80

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 74
variable_sites: 3
pi: 0.0405
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TCTTCTTCCGAGAACCACCT	TCGCCATCTCCATATCTGGT	50.0	50.0	58.0	58.0	228 bp	1.0	80.8
psbT-psbN_p2	TTCCGAGAACCACCTAAGGT	TCGCCATCTCCATATCTGGT	50.0	50.0	58.0	58.0	223 bp	1.0	80.8
psbT-psbN_p3	TCTTCTTCCGAGAACCACCT	CCATTCGAAGAACATGGGGA	50.0	50.0	58.0	57.6	139 bp	1.0	78.3
psbT-psbN_p4	TCTTCTTCCGAGAACCACCT	GTCGCCATCTCCATATCTGG	50.0	55.0	58.0	57.6	229 bp	1.0	77.9
psbT-psbN_p5	TTCCGAGAACCACCTAAGGT	GTCGCCATCTCCATATCTGG	50.0	55.0	58.0	57.6	224 bp	1.0	77.9