Hotspot

psbE-petL

LSC · 63749-64986 · 1238 bp

Back to Stenotaphrum

Pi 0.018

MarkerSeek score 56.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1238
variable_sites: 22
pi: 0.018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	TTTATGGGCAGTAGTGGGGA	45.0	50.0	58.0	58.0	2053-2078 bp	1.0	81.9
psbE-petL_p2	CAACCCGCAATGAATAGGGA	TTTATGGGCAGTAGTGGGGA	50.0	50.0	57.9	58.0	2042-2067 bp	1.0	81.6
psbE-petL_p3	TGACAAATAACCAACCCGCA	ACGCAGTCACGAATAATCCC	45.0	50.0	58.0	58.1	1703-1740 bp	1.0	81.3
psbE-petL_p4	GTGGGTACAGCTAATCCGTG	TTTATGGGCAGTAGTGGGGA	55.0	50.0	58.1	58.0	2271-2296 bp	1.0	81.3
psbE-petL_p5	TGACAAATAACCAACCCGCA	TTTGAGAGTTGAGAGCGCTC	45.0	50.0	58.0	57.9	1956 bp	0.5	59.3